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Encompassing Amino Acids: 19-158
Application: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Description: Mouse CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_010581.3, a.a. 19-158 fused at the C-terminus to the Fc portion of human IgG1 and C-terminal Avi-tag expressed in a HEK293 cell expression system. The protein is enzymatically biotinylated using AviTag™ technology. MW = 44 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_010581.3
Storage Stability: At least 6 months at -80°C (for all proteins)
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-Tag
Uniprot: Q08722
Warnings: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Vernon-Wilson, E.F., et al., Eur. J. Immunol. 2000; 30(8): 2130-21375.2. Majeti, R. et al., Cell. 2009; 138(2): 286-299.
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