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Applications: Ideal for protein binding analyses and studying SIRPα receptor activity.
Background: CD47 (also known as Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin,) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on macrophages. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, creating a "don't eat me" signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells, where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance.
Description: Recombinant HEK293 cell line over-expressing full length human CD47 (NM_198793.2). Surface expression is confirmed by flow cytometry.
Format: Each vial contains ~3 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell Line: HEK293
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1.Ide K et.al. (2007) Role for CD47-SIRPα signaling in xenograft rejection by macrophages. PNAS 104: 5062-5066.2.Jaiswal S et.al. (2009) CD47 is upregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis. Cell 138: 271-285.
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