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Applications: This kit is useful for screening for inhibitors of CD47 binding to SIRP-α.
Background: Signal-regulatory protein alpha (SIRP-α), also known as CD172a, is a cellsurface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, isubiquitously expressed on the surface of all cells and has been found to be overexpressed insome cancers. CD47 binding to SIRP-α delivers a "Do Not Eat Me" signal to macrophages. Inthe context of a tumor, this signaling aids in tumor evasion of the immune system. Inhibitors ofthis interaction hold great promise for cancer immunotherapy.
Description: The CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-α-biotin is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96- well format, with biotin-labeled SIRP-α, purified CD47, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chao, M.P., et al., Curr. Opin. Immunol. 2012; 24(2): 225-232.
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