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Encompassing Amino Acids: 29-215
Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Concentration: 0.87 mg/ml
Description: Human secreted CD58-Fc fusion protein, also known as LFA-3, GenBank Accession No. NM_001779, a.a. 29-215, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Genbank: NM_001779
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: P19256
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wang, J., et al., Cell. 1999 Jun; 97(6):791-803.2. Raychaudhuri, S., et al., Nature. 2009 Nov ;41:1313-1318.
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