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Encompassing Amino Acids: 27-547
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
Description: Human CD73, also known as 5'-nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW = 61 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_002526
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: C-terminal Avi-His-tag
Uniprot: P21589
Biosafety Level: Not applicable (BSL-1)
References: 1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.
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