Target: CD74
Conjugate: ATTO 594
Product Type: Monoclonal
Clone Number: 3D7
Immunogen: Human CD74 invariant chain synthetic peptide.
Swiss-Prot: P04233
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~33 kDa protein.
Cellular Localization: Cell Membrane,Endoplasmic Reticulum,Endoplasmic Reticulum Membrane,Golgi Apparatus,Endosome,Lysosome
Scientific Background: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment.Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
References: 1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9.3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816.4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11.5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165.6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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