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Description: Complex of human CDK13 (cyclin dependent kinase 13) and human cyclin K. The proteins were co-expressed in Sf9 insect cells along with S. cerevisiae CAK1 (Serine/threonine-protein kinase CAK1).Recombinant human CDK13, encompassing amino acids 516-1512 (end), contains an N-terminal GST-tag. Recombinant human Cyclin K, full length encompassing amino acids 1-580 (end), also contains an N-terminal GST-tag. Recombinant S. cerevisiae CAK1, encompassing amino acids 2-368 (end), contains a FLAG-tag. The proteins were purified using the GST tags.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Genbank: CDK13: NM_003718; Cyclin K: NM_001099402
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: CDK13: Q14004; Cyclin K: O75909
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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