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Encompassing Amino Acids: full length
Applications: Useful for three dimensional structure studies, binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 50 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Complex of N-terminal GST-tagged recombinant full length human CDK3 (GenBank Accession No. NM_001258), and Cyclin E1 (GenBank Accession No. NM_001238). Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK3 MW=61 kDa, Cyclin E1 MW=74 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Genbank: NM_001258 (CDK3), NM_001238 (Cyclin E)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q00526 (CDK3), P24864 (Cyclin E)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bullrich, F. et al., Cancer Res. (1995); 55:1199-1205.
2. Ye, X. et al., Proc. Nat. Acad. Sci. (2001); 98:1682-1686.
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