Application Note: Anti-CDK4 is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 85 mg/mL
ELISA Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:200 - 1:500
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: CDK4 (p34) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical State: Liquid (sterile filtered)
Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 34 kDa cdk4 (p34) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk4 (p34) from other species has not been determined.
Background: Cyclin-dependent kinase 4 is a Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. It hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. It also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Cdk4 is a component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Low Endotoxin: No
Other: User Optimized
English
Deutsch
