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Encompassing Amino Acids: full length
Applications: Useful for three dimensional structure studies and binding assays.
Description: Complex of recombinant, full length human CDK4 (GenBank accession number NM_000075) with C-terminal His-tag and modifications (a.a. 43G-47G were replaced with EE, and T172A), and human Cyclin D1 (GenBank accession number NM_053056), a.a. 16-271, were co-expressed in a baculovirus-infected Sf9 cells expression system. CDK4 MW=34 kDa, Cyclin D1 MW=29 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
Genbank: NM_000075(CDK4), NM_053056(cyclin D1)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag (CDK4), no tag on Cyclin D1
Uniprot: P11802 (CDK4), P24385 (cyclin D1)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Amendola D. et al., Cell Prolif. 2009. Feb; 42(1):94-109.
2. Yang L. et al., Biochem Cell Biol. 2009. Dec; 87(6):933-42.Application Reference(s):
1. Iterative conversion of cyclin binding groove peptides into druglike CDK inhibitors with antitumor activity (2015)
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