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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase- Glo reagent (Promega).
Description: Complex of N-terminal Histaggedrecombinant full-length humanCDK6 (GenBank Accession No.X66365), and N-terminal His tagged,recombinant full length, Cyclin D3.(GenBank Accession No. M90814).Coexpressed in a Baculovirus infectedSf9 cell expression system. CDK6MW=38 kDa, Cyclin D3 MW=33 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, 110 mMNaCl, 2.2 mM KCl, 300 mM Imidazole, 3mM DTT and 20% glycerol
Genbank: NM_001259/NM_001760
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tags
Uniprot: Q00534 (CDK6); P30281 (Cyclin D3)
Warnings: Avoid freeze/thaw cycles. Storing diluted enzymeis not recommended.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gauduchon J. et al., Int J Cancer. 2008 May 1,122(9):2130-41.
2. Faast R. et al., Oncogene. 2004 Jan 15,23(2):491-502.