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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: CDK6 is a serine/threonine protein kinase involved in cell cycle progression and in cellular differentiation. The CDK6/Cyclin D3 complex phosphorylate RB1 to release the transcription factor E2F and drive the transition from G1 to S-phase of the cell cycle. This complex regulates cell proliferation in many cell types and has been validated as a cancer therapeutic target. Palbociclib, a CDK4/CDK6 inhibitor, has shown therapeutic benefit in a small number of patients and is in clinical use for the treatment of ER+/HER2- breast cancer, with more clinical trials ongoing.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The CDK6/CyclinD3 Kinase Assay Kit is designed to measure CDK6/CyclinD3 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK6/CyclinD3 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Uniprot: Q00534
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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