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Encompassing Amino Acids: 430-543
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: CeCR2 was incubated with various amounts of BET ligand in assay buffer in a 10 µl reaction for 30 min at RT. Reactions with H4 1-21[B] were prepared in parallel as control. AlphaLISA® Ni acceptor beads (1:250) were added and incubation continued for 30 min more. Finally, Streptavidin Donor beads (1:125) were added and mixture was incubated for 10 min at RT before A-counts were measured.
Description: Human Bromodomain CECR2 (cat eye syndrome chromosome region, candidate 2), GenBank Accession No. NM_031413, a.a. 430-543 corresponding to single bromodomain with N-terminal His-tag, MW = 14.4 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_031413
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9BXF3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chen J, et al.,Int J Dev Biol. 2010;54(5):925-9.
2. 2. Lee SK, et al., Mol Cells. 2012 Jul;34(1):85-91.
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