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Application: Use in time-course and endpoint cellular viability assays.Use as ER-positive xenograft cell line for fluorescence imaging for bioluminescence imaging.
Background: MCF7 is a breast cancer cell line derived from a pleural effusion metastatic site. MCF7 is a hormone receptor positive (HR+) breast cancer cell line that expresses high levels of both estrogen receptor (ER) and progesterone receptor, with low expression of human epidermal growth factor receptor 2 (HER2). This receptor expression pattern renders MCF7 cell line useful as a model to develop hormone receptor targeting therapeutics and as a target-negative cell line for the development of therapeutics against HER2.This cell line constitutively expresses both eGFP and firefly luciferase, making it ideal for both time-course and endpoint cellular assays.
Description: eGFP/Firefly Luciferase MCF7 Cell Line is a MCF7 human breast mammary gland cell line engineered to express eGFP (enhanced green fluorescence protein) and Firefly luciferase driven by a CMV promoter. This cell line was generated by transduction with Firefly Luciferase-eGFP Lentivirus (#79980-P).
Host Cell Line: MCF7 cells, human breast mammary gland cell line, epithelial cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Horwitz, et al., 1975 Steroids 26 (6): 785-795.Lee, et al., 2015 J Natl Cancer Inst. 107 (7): djv073.Singh, et al., 2016 J Pharmacokinet Pharmacodyn. 43(6):567-582.
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