The PYROGENT–5000 Kinetic Turbidimetric LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the incubating absorbance plate reader, and automatically monitored over time (via WinKQCL Software) for the appearance of turbidity. In the presence of endotoxin, the lysate will begin to gel, causing the solution to become cloudy or turbid. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration in unknown samples can be calculated from a standard curve. The PYROGENT –5000 Kinetic Turbidimetric Assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENT –5000 Kinetic Turbidimetric Assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. For PYROGENT -5000 Bulk Kinetic Turbidimetric Kits, kinetic turbidimetric LAL, reconstitution buffer and matched control standard endotoxin are packaged separately but should be ordered together.
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