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Background: B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules.
Description: The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB-luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by four copies of the NF-κB Response Element upstream of a minimal promoter. Upon ligand binding, active BCMA will initiate the NF-κB signaling pathway, leading to expression of the NF-κB-controlled luciferase reporter.
Host Cell Line: HEK293
Mycoplasma Testing: This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Storage Stability: Store in liquid nitrogen immediately upon receipt. Do not store long-term at -80°C or on dry ice.
Supplied As: Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: BSL-2
References: Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
Yu, G. et al. (2000) Nat. Immunol. 1:252.
Yan, M. et al. (2000) Nat. Immunol. 1:37.
Gravestein, L.A. and Borst, J. (1998) Sem. Immunol. 10:423.
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