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Applications: This cell line is useful to stimulate human CD137 (4-1BB) on immune cells, and for use in binding assays for CD137.
Background: CD137L (4-1BBL; TNLG5A), a part of the tumor necrosis factor (ligand) superfamily (TNFSF9), is a type II glycoprotein that expresses on various antigen-presenting cells. It interacts with CD137 (4-1BB) on T cells following TCR stimulation to promote T cell expansion and cytokine production.
Description: Recombinant CHO-K1 cell constitutively expressing full-length human CD137L (NP_003802.1). Surface expression is confirmed by flow cytometry.
Format: Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell Line: CHO-K1
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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