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Applications: Compound or antibody screening Functional assays for CFTR Antigen preparation for mouse immunization
Background: Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Biological Activity: The expression of CFTR is confirmed by Western blotting and flow cytometry (FACS).
Description: The CFTR HEK293 Cell Line expresses full length, wild-type human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank #P13569, NP_000483.3), with a C-terminal Streptavidin-Binding Peptide (SBP) tag.Expression must be induced ≥24 hours prior to an experiment using 1 µg/ml Doxycycline and 3 mM Na-butyrate. The inducible expression of CFTR was confirmed by Western blotting and flow cytometry.
Genbank: P13569
Host Cell Line: HEK293
Storage Stability: Store in liquid nitrogen immediately upon receipt. Cells are stable after a minimum of seven continuous passages. Upon first use, amplify the cells in culture and make several frozen aliquots for future use.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of Sigma Freezing Medium (Cat. # C-6164).
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).2. Hillier, L.W., et al. Nature 424:157-164 (2003).3. McCann, C. M., et al. BioTechniques 38 (6):945–952 (2005).
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