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Applications: Study the consequences of losing MHC class II.Study T cell activation, antigen presentation, and immune responses.Useful for the development of improved universal CAR-T or other effector cells.
Background: CIITA (Class II Transactivator, also known as Class II Major Histocompatibility Complex Transactivator) acts as a coactivator for MHC class II-specific gene expression, and negatively regulates IL-4 expression during T cell differentiation. IFN-Ƴ induces CIITA gene expression via the JAK1 and STAT1 pathways. The GTP-binding and acidic, proline-serine-threonine rich regions of CIITA appear to be required for its activity. Defects in CIITA have been implicated in Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.
Description: CIITA (Class II Transactivator) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.
Host Cell Line: THP-1, monocyte, suspension
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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