eGFP Daudi Cell Line

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Applications: In vitro and in vivo fluorescence imaging.Use as target cells in CAR-T co-culture killing assays.

Background: Daudi is a B lymphoplast cell line isolated from a human Burkitt lymphoma patient, which is commonly used in the study of leukemia. Burkitt lymphoma is a type of B-cell non-Hodgkin lymphoma, which is a rare and fast-growing cancer with origins often in the abdomen or spleen. This cell line is used as target cells in studies involving T cells. The presence of eGFP (enhanced green fluorescent protein) allows for easy assay readouts.

Description: eGFP Daudi Cell Line is a Daudi cell line engineered to express enhanced GFP (eGFP) driven by an EF1a promoter. This cell line was generated by transduction with Enhanced GFP Lentivirus (Puromycin) (BPS Bioscience #78639).This cell line has been validated by flow cytometry for eGFP expression.

Host Cell Line: Daudi

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: ˃1 x 10⁶ cells in 1 ml of of Cell Freezing Medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• SKU: BPS82297
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82297
• Green Labware: No
• Package Unit: 2 vials
• Quantity Unit: PAK