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Applications: Use as target cells in CAR-T or NK co-culture killing assays.In vitro and in vivo bioluminescence imaging studies.
Background: U-87 MG is an epithelial cell line isolated from a malignant glioma and it is commonly used in neuroscience and glioblastoma research. The tumor suppressor gene PTEN (phosphatase and tensin homolog) is deleted in the U-87 MG glioblastoma cell line resulting in hyperactivation of the phosphoinositide 3-kinase (PI3K) pathway, which leads to sustained PI(3,4,5)P3 signaling, and thereby hyperactivation of Akt (protein kinase B) and other effectors. Therefore, PTEN-negative U-87MG glioblastoma cells are widely used to study the modulation of the PI3K/Akt/GSK-3β pathway.
Description: eGFP/Firefly Luciferase U-87 MG Cell Line are engineered human glioblastoma U-87 MG cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.
Host Cell Line: U-87 MG, human glioblastoma cell line, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Allen M., et al., 2016 Trans. Med. 8(354): 1-4.Moon S.H., et al., 2013 Int J Oncol. 42(3):921-8.
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