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Applications: Screen for antibodies binding to CD16A or examine CD16A function in a cellular context
Background: Fc Gamma Receptor 3A (FcGRIIIA; FcγRIIIA), also known as CD16A, is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity, and clearance of immune complexes. The human FcGR3A displays a dimorphism at residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158 while the other allele (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.FcGR3A (CD16A) plays a role in the activation of natural killer (NK) cells. Clinically, it serves as a marker of certain immune cells such as neutrophils. Its expression has been observed at the surface of T cells in patients with chronic viral infections (such as COVID-19). It is considered a potential therapeutic target to potentiate the efficacy of therapeutic antibodies used to treat solid tumors, or as direct target in hematopoietic cancers.
Description: Recombinant CHO cell line stably expressing full length human CD16A (also known as FCGR3A, FcγRIIIa; ref. seq. NM_ 000569.8) under the control of the CMV promoter.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Thaw Medium 3 (BPS Bioscience #60186):
Ham's F-12 medium supplemented with 10% FBS, 1% Penicillin/StreptomycinGrowth Medium 3J (BPS Bioscience #79974):
Ham's F-12 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin and 5 µg/ml Puromycin
Genbank: NM_ 000569.8
Host Cell Line: CHO
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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