Firefly Luciferase MC38 Cell Line

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Applications: Use as an internal control in CAR-T or NK co-culture killing assaysIn vitro and in vivo bioluminescence imaging

Background: The MC38 cell line was derived from a colon adenocarcinoma from a C57BL/6 mouse. The cells are adherent and have a fibroblast morphology. MC38 cells form tumors and metastases following implantation into syngeneic C57BL/6 mice or immunocompromised mice. The signal generated by the firefly luciferase is proportional to cell numbers.

Description: Recombinant MC38 cells constitutively expressing the firefly (Photinus pyralis) luciferase gene under the control of a CMV promoter.

Host Cell Line: MC38

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS78383
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78383
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Mouse