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Applications: Monitor IDO2 pathway activity. Screen for activators or inhibitors of IDO2 in a cellular context.
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells.The L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp. Additionally, the released degradation products of L-Trp have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumorsand provide cancer cells with an avenue for immune evasion.
Description: Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294.
Format: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO
Genbank: NM_194294
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Metz, R., et al., Int. Immunol. 2014; 26: 357-367. 2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.
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