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Applications: This cell line is useful for LIGHT binding assays, flow cytometry, or for screening for LIGHT antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with HVEM/NF-κB-luciferase/Jurkat reporter cell line (BPS Bioscience).
Background: LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTβR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-κB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface.
Description: Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry.
Host Cell Line: CHO
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Chen, et.al. (2013) Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 2013 April ; 13(4): 227-242.2. Steinberg, et.al. (2014) The Signaling Networks of the Herpesvirus Entry Mediator (TNFRSF14) in Immune Regulation. Immunol Rev. 2011 November; 244(1): 169-187.3. Rio, et.al. (2014) Therapeutic blockade of LIGHT interaction with HVEM and LTβR attenuates in vivo cytotoxic allogeneic responses. Transplantation. 2014 December 15; 98(11): 1165-1174.
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