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Applications: Monitor Myc pathway activity and screen for activators or inhibitors of the Myc pathway.
Background: The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizeswith Max to regulate transcription of genes involved in proliferation, transformation andangiogenesis. Myc mutations have been linked to the development of a number of humancancers, including Burkitt's lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma,making Myc a promising therapeutic target.
Description: The Myc Reporter - HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into HCT116 cells, a human colon cancer cell line. HCT116 contains a mutated beta-catenin which leads to the accumulation of β-catenin and constitutive activation of downstream Myc that induces the expression of Myc luciferase reporter. The cell line is validated for the inhibition of the expression of Myc luciferase reporter.
Host Cell Line: HCT116
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Supplied As: Each vial contains 1.5 X 10^6 cells in 1 ml of 10% DMSO.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: Pelengaris S, et al. (2002) c-MYC: more than just a matter of life and death. Nat. Rev.Cancer. 2(10): 764-76.
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