Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen for activators or inhibitors of NF-κB signaling pathway.
Screen for activators or inhibitors of RANKL/RANK pathway.
Description: The NF-κB reporter (Luc)-Raw 264.7 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line: Raw 264.7
Mycoplasma Testing: The cell line has been screened using the PCR-based VenorGeM™ Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains 2 X 10^6 cells in 1 ml of 10% DMSO.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Peninnger, J. M., et al. (2006) RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol. Med. 12(1):17-25.
2. Baeuerle, P.A. (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.
English
Deutsch
