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Description: The NF-κB reporter (Luc)-HCT-116 cell line is designed to monitor nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Format: Aqueous solution containing DMSO
Host Cell Line: HCT116
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Biosafety Level: BSL-2
References: 1. Samuel T et.al. (2014) Variable NF-B pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.
2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-B pathway. Nature Communication
3: 976. 3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-B activity. Carcinogenesis 29: 849.
4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-B. Acta Naturae 3: 77.
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