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Applications: Screen for activators or inhibitors of NFAT signaling pathwayDetermine T-cell activation through T-cell receptor (TCR) or Chimeric Antigen Receptor (CAR)
Background: The Nuclear Factor of Activated T-Cells (NFAT) family of transcription factors plays an important role in mediating the immune response. T-cell activation through the T-cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase Calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal (NLS) promoting NFAT nuclear translocation and inducing gene expression, including various cytokines (IL-2, IL-3, IL4, and TNF-alpha). Members of the NFAT family have been found in many tissue types, including the heart, skeletal muscle and brain. This reporter cell line is designed to monitor T-cell activation or inhibition through various checkpoint inhibitors. It can be used as a control or parental cell line to co-express various immune checkpoint inhibitors, such as PD1.
Description: Recombinant Jurkat T-cells expressing an enhanced GFP (eGFP) gene under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining eGFP expression.
Host Cell Line: Jurkat
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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