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Applications: Screen for compound effect on the MAPK/ERK signaling pathway.
Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
Background: The MAPK/ERK signaling pathway is a key regulator of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The Ternary Complex Factors (TCFs), including Elk1, are among the best-characterized transcription factor substrates of ERK1/2. When phosphorylated by ERK1/2, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
Description: Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway.
Formulation: Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS, 1% non-essential amino acids (Corning, #25-025-CI), 1 mM Na pyruvate (Corning, #25-000-CI), 1% Penicillin/Streptomycin (Thermo Fisher, #15140163)Growth Medium 1J (BPS Bioscience #79552):
Thaw Medium 1 (BPS Bioscience #60187), 500 µg/ml of Geneticin/G418Assay Medium 1: Thaw Medium 1 (BPS Bioscience #60187)Assay Medium 1B (BPS Bioscience #79617): MEM medium (Thermo Fisher, #11095098) supplemented with 0.5% FBS, 1% non-essential amino acids (Corning, #25-025-CI), 1 mM Na pyruvate (Corning, #25-000-CI), 1% Penicillin/Streptomycin (Thermo Fisher, #15140163)
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Purity: SRE cell line, MAPK reporter, ERK reporter
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.
Supplied As: 2 vials: Each vial contains 2 x 106 cells in 1 ml of 90% FBS, 10% DMSO
Warnings: Avoid freeze/thaw cycles. Do not use a -80°C freezer for long term storage.
Biosafety Level: BSL-2
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