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Applications: Characterize the biological activity of KIR3DL3 or TMIGD2 interactions with B7-H7
Screen for B7-H7 antibodies
Description: Recombinant CHO-K1 cells constitutively expressing human B7-H7 (also known as HHLA2: HERV-H LTR-associating protein 2, GenBank accession #NM_007072) and an engineered T cell receptor (TCR) activator. B7-H7 mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2) in naÍve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 3A (BPS Bioscience #60188):
F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin and 500 µg/ml of Hygromycin.Media Required for Cellular Assay
Thaw Medium 2 (BPS Bioscience #60184): RPMI1650 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.
Genbank: NM_007072
Host Cell Line: CHO
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Bahatt RS, et. al., Cancer Immunol Res. 2021 Feb;9(2):156-169.
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