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Applications: Screening or performing binding assays of antibodies against human CD2 in a cellular context.
Background: CD2 (Cluster of differentiation 2, also known as T11, SRBC, or LFA-2) is a cell adhesion molecule and surface antigen found on the surface of all peripheral blood T-cells and natural killer (NK) cells. It is used as specific marker of T and NK cells. CD2 interacts with LFA3 (CD58) on antigen-presenting cells to optimize immune recognition. It also functions as a co-stimulator in T and NK cells. It is being investigated as a therapeutic target for the treatment of T cell lymphomas and leukemias.
Description: Recombinant clonal CHO-K1 stable cell line constitutively expressing full length human CD2 protein. The surface expression of CD2 in this cell line was validated by flow cytometry.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 3J (BPS Bioscience #79974):
F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 µg/ml Puromycin.
Genbank: NM_001767
Host Cell Line: CHO
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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