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Application: Screen for activators or inhibitors of antibody-mediated signaling for immunotherapy research and drug discovery.
Characterize CD5 antibodies and ligands.
Background: CD5 is a type-1 transmembrane glycoprotein with an extracellular region composed of three scavenger receptor, cysteine-rich domains. This lymphocytic-specific scavenger receptor functions at the interphase of the innate and adaptive immune responses. It is involved in: (i) microbial-associated pattern recognition; and (ii) modulation of intracellular signals mediated by antigen-specific receptor present in T and B cells. CD5 is expressed by all T cells and some B cell subsets (B1a and Breg or B10). T cells usually express higher levels of CD5 than B cells, and CD5 is upregulated in T cells upon strong activation. CD5 is a signaling co-receptor, associated with the antigen-specific receptor complex of T and B cells, and down-modulates the activation/differentiation signals delivered upon specific antigen recognition. The signaling pathway used by CD5 is only partially known.
Description: Recombinant CD5-CHO K1 cell line stably expressing a full-length human CD5 receptor (accession number: NM_014207.3). Surface expression of hCD5 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hCD5 expression (High, Medium and Low) to mimic different stages of cancer target cells with various CD5 expression levels.
Formulation: Thaw Medium 3 (BPS Bioscience #60186): F-12K medium (Hyclone, #SH30526.01) supplemented with 10% FBS, 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)Growth Medium 3B (BPS Bioscience #79529):
Thaw Medium 3 (BPS Bioscience, #60186) plus 500µg/ml of Hygromycin B.
Genbank: NM_014207.3
Host Cell Line: CHO-K1
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ~2 x 106 cells in 1 ml of 90% FBS, 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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