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Applications: Monitor cAMP/PKA signaling pathway activity.Screen for activators or inhibitors of cAMP/PKA signaling pathway.
Background: Cyclic adenosine monophosphate (cAMP) is a second messenger involved in cell signaling that regulates various physiological and pathological processes. cAMP regulates the transcription of target genes by activating protein kinase A (PKA) and the transcription factor cAMP response element-binding protein (CREB), a downstream effector. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors), and neurotrophins. In the cAMP/PKA signaling pathway, CREB is activated via PKA-mediated phosphorylation and binds to CRE with a general motif of 5'-TGACGTCA-3'. The cAMP/PKA/CREB signaling pathway has both tumor-suppressive and tumor-promoting effects in cancer cells and can be useful in studying cancer signaling pathways.
Description: The CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway) contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into CHO cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to Forskolin.
Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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