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Applications: Useful as MSLN-expressing target cells in co-culture assays with MSLN CAR-T cells and for screening other MSLN-binding molecules.
Background: Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently over-expressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy approaches, including drug-conjugated antibodies and chimeric antigen receptor T-cells (CAR-T Cells).
Description: Recombinant CHO-K1 cells expressing cleaved, mature human Mesothelin (MSLN) gene (GenBank Accession #NM_005823.5) under the control of cytomegalovirus (CMV) promoter. This cell line was generated by limited dilution and isolation of individual clones, which were screened based on MSLN expression to obtain a high-expressing cell line.
Host Cell Line: CHO-K1
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: 2 vials of frozen cells. 2 x 10^6 in 1 ml of 90% FBS, 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Asgarov, K., et al. (2017). A new anti-mesothelin antibody targets selectively the membrane-associated form. MAbs, 9(3): 567-577. https://doi.org/10.1080/19420862.2017.1288770.
2. Ye, L., et al. (2019). Mesothelin-targeted second generation CAR-T cells inhibit growth of mesothelin-expressing tumors in vivo. Experimental and Therapeutic Medicine, 17: 739-747. https://doi.org/10.3892/etm.2018.7015
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