MART-1 TCR (DMF5) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

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Applications: Design and optimize co-culture bioassays for MART-1-specific TCR cell evaluation.Use as a positive control in experiments evaluating MART-1-specific TCR expressing cells.

Background: MART-1 is a differentiation antigen expressed on the surface of melanocytes. A peptide fragment of the protein is found in MHC complexes that are recognized by CD8⁺ cytotoxic T cells. MART-1 is present in most skin cancers, including melanomas, and is used as a biomarker for diagnostic purposes. Since the expression of the protein is restricted to melanocyte containing tissues (skin and retina), and is not found in other tissues, MART-1 is an attractive target for cancer vaccines and adoptive cell therapy. The MART-1 peptide 26-35 is a fragment commonly associated with MHC and recognized by T cell receptors.CD8 (Cluster of Differentiation 8) is a co-receptor of TCR and a typical marker of cytotoxic T cells. The TCR protein complex is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of Activated T-cells) transcription factors that induce the expression of various cytokines such as interleukin-2 to 4, and TNF-alpha. The use of engineered TCR allows T cells to target specific antigens present in cancer cells, via the MHC, expanding the portfolio of antigens that can be targeted in cancer cell therapy. 

Description: MART-1 TCR (DMF5) CD8⁺ NFAT-Luciferase Reporter Jurkat Cell Line is a Jurkat cell line generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and MART-1-specific TCR (DMF5) using lentiviral transduction (with CD8a Lentivirus #78648 and MART-1-Specific TCR Lentivirus (Clone DMF5) #78679). The human TCR clone DMF5 specifically recognizes tumor antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A).

Host Cell Line: Jurkat (clone E6-1)

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Uniprot: Q16655

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• SKU: BPS78773
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78773
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human