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Applications: Screen and validate antibodies against MUC16 for drug discovery and research.Perform binding assays to screen for potential MUC16 ligands.
Background: MUC16 (also known as Mucin16, Cell Surface Associated, and ovarian cancer marker CA125) is a protein of the mucin family. Mucins are high molecular weight, O-glycosylated proteins found on the apical surfaces of epithelia. MUC16 has been found in the cornea, the respiratory system, and the female reproductive system, where it plays a role in protecting epithelial cells from pathogen entry. MUC16 dysfunction has been associated with dry eye, cystic fibrosis and cancer, in particular ovarian cancer. MUC16 binds to mesothelin and may be involved in migration and metastasis of tumor cells. Currently MUC16 is used as biomarker for certain cancer types, in addition to being studied in the context of cell signaling. Due to its high expression in some tumors, it is investigated as a therapeutic target for antibody-based therapy as well as adaptive cell therapy.
Description: Engineered CHO cell line stably expressing human C-terminus MUC16 (CA125) transcript variant 4, from amino acid 13785 to 14507 (NM_024690.2). This portion of MUC16 contains several of the extracellular SEA modules, the transmembrane domain, and the short cytoplasmic tail of the protein. Surface expression of MUC16 was confirmed by flow cytometry. This stable clonal cell line was selected based on its high level of MUC16 expression compared to the parental CHO-K1 cell line.
Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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