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Application: Study phenotypes resulting from Nectin-4 knockout.Use as negative control in the development of ADC or other biologics targeting Nectin-4.
Background: Nectin-4 is part of the Nectin family and plays a crucial role in cell adhesion. Nectin-4 contains two immunoglobulin-like C2-type domains and one Ig-like V-type domain. In contrast to other Nectins, which are found extensively in adult tissues, Nectin-4 is abundant during fetal development but declines in adult life. Its expression, however, returns specifically in lung, breast, pancreas and ovarian cancers. It was shown that Nectin-4 can modulate the expression of epithelial-mesenchymal transition-related proteins via the PI3K (phosphoinositide 3-kinase)/AKT (protein B kinase) pathway. In addition, a recent study demonstrated that Nectin-4 is a cancer-specific TIGIT ligand, and its expression is also associated with poor prognostic features, suggesting it could be an efficient target for cancer immunotherapy. In 2019, the FDA approved Enfortumab vedotin-ejfv, which is a Nectin-4-directed antibody drug conjugate (ADC) used in treating metastatic urothelial cancer.
Description: Nectin-4 Knockout MCF7 Cell Line is an MCF7 breast cancer cell line in which human Nectin-4 has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human Nectin-4.This cell line has been validated by genomic sequencing and flow cytometry.
Host Cell Line: MCF7 human breast mammary gland cell line. Adherent epithelial cells
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: Q96NY8
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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