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Application: Screen therapeutic antibodies and antibody drug conjugates (ADCs) targeting PTK7Co-culture assays with PTK7 targeting CAR-T cells
Background: PTK7 (Protein Tyrosine Kinase 7) is a pseudokinase of the receptor tyrosine kinase (RTK) family of Wnt receptors and shares a common ligand (Wnt5a) with fellow pseudokinases ROR1 (receptor tyrosine kinase-like orphan receptor 1) and ROR2. Despite the lack of a functional kinase domain PTK7 still contributes to downstream signaling of Wnt5a through its interaction with LRP6 (low-density lipoprotein receptor-related protein 6) and FZD (frizzled) receptors. PTK7 expression contributes to oncogenic potential in a variety of solid tumors including ovarian, colorectal and breast cancers and neuroblastoma. The cell surface expression and oncogenic function of PTK7 in a variety of tumor types makes it a compelling target for both CAR (chimeric antigen receptor)-T cell and antibody based therapeutic development. The use of cofetuzumab pelidotin, an ADC (antibody-drug conjugate) based on auristatin and targeting PTK7, showed promise in the treatment of ovarian cancer, NSCLC (non-small cell lung cancer) and TNBC (triple-negative breast cancer), showing the potential of targeting PTK7 in oncology.
Description: PTK7 CHO Cell Line is a CHO-K1 cell line expressing PTK7 (Protein Tyrosine Kinase 7) (NM_002821.5) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lentiviral transduction followed by puromycin selection and limited dilution. Individual clones were screened for PTK7 expression levels by flow cytometry and a high expressing clone was selected to generate this cell line.
Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: Dessaux C., et al., 2024 Oncogene 43, 1973–1984.Jie Y., et al., 2021 Front. Immunol. 12:665970.Lee J.Y., et al., 2023 Cell Rep Med 4(6):101091.Maitland M.L., et al., 2021 Clin Cancer Res 27 (16): 4511–4520.
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