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Applications: Screen for inhibitors of RANKL/RANK signaling in a cellular context
Background: RANK/RANKL/OPG pathway plays a significant role in osteoclast maturation, which are the major cells in charge of bone resorption. If RANKL binds to the receptor RANK instead of binding to its decoy receptor Osteoprotegerin (OPG), it activates the NF-κB pathway to promote osteoclast survival and proliferation, resulting in the resorption of bone. Therefore, abnormal production of RANKL or OPG can cause osteoporosis and other bone-related disorders, making the RANKL/RANK signaling pathway a valuable target for drug development.
Description: Recombinant HEK-293 cells expressing firefly luciferase gene under the control of Nuclear factor-κB (NF-κB) with constitMedium 1Gutive expression of human RANK (Receptor activator of nuclear factor-κB; TNFRSF11A; ref. seq. NM_003839.2).
Genbank: NM_003839.2
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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