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Application: Use as a negative control for Cas9 Expressing iPS Cell Pool (#78578).Validate and use as control in customer specific applications prior to utilizing genetically engineered iPS cell pools/cell lines.
Background: The discovery by Yamanaka and colleagues in 2007 that 4 factors were sufficient to reprogram terminally differentiated fibroblasts into pluripotent stem cells launched the advent of human induced pluripotent stem (iPS) cell technology. These human iPS cells are capable of both self-renewal and differentiation down all three germline lineages and provide both a tool to model human development and disease in the relevant differentiated human cell types, and a unique opportunity for high throughput drug screening and cell therapy development. The use of a control cell line that underwent similar manipulation processes is considered essential to guarantee that any change in cell behavior or differentiation potential observed using Cas9 Expressing iPS Cell Pool is not due to Cas9 overexpression.
Description: The Transduction Control (EF1A Promoter) iPS Cell Pool is an iPS (induced pluripotent stem) cell pool that was generated via lentiviral transduction with Expression Negative Control Lentivirus (EF1A Promoter/Puromycin Resistance) (#82212-P). This cell pool can be used as control for Cas9 Expressing iPS Cell Pool (#78578).
Host Cell Line: PBMC-derived, non-Disease Human iPS Cell Line (iXCells 30HU-002)
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the cell population over time. Clonal selection can be performed. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of iPS Cell Freezing Medium
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Takahashi K., et al., 2007 Cell. 131(5):861-872.
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