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Application: Use as a negative control when testing TROP2 inhibitors in MCF7 cells.Study phenotypes resulting from TROP2 knockout.
Background: TROP2 (Trophoblast cell-surface antigen 2), also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein that is highly expressed in a variety of solid cancers yet has low expression in normal cells. Through a variety of signaling pathways, such as the MAPK (mitogen-activated protein kinase)/PI3K (phosphoinositide 3-kinase)/AKT (protein kinase B) pathway TROP2 regulates cancer growth and metastasis. Overexpression of this protein is associated with a poor prognosis in cancers such as breast, gastric and ovarian cancer. In other cases, such as NSCLC (non-small cell lung cancer) lower expression seems to link to the malignancy. TROP2 is a favorable target for antibody drug conjugates (ADC) and immunotherapy. Studies with Sacituzumab govitecan, an ADC composed of an anti-TROP2 humanized monoclonal antibody and SN-38 (topoisomerase I inhibitor), in cell models have shown promising results.
Description: TROP2 Knockout MCF7 Cell Line is an MCF7 breast cancer cell line in which human TROP2 (Trophoblast cell-surface antigen 2 or TACSTD2) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human TROP2.This cell line has been validated by genomic sequencing and flow cytometry.
Host Cell Line: MCF7 human breast mammary gland cell line. Adherent epithelial cells
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: P09758
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Lombardi P., et al., 2023 Cancers (Basel) 15 (6): 1744.
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