TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line

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Applications: Characterize the biological activity of TMIGD2 interaction with B7-H7Screen for B7-H7 antibodies in co-culture with B7-H7-expressing cells

Background: HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 in naive T cells while it delivers an immune-inhibitory signal through KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3) in activated T cells and Natural Killer (NK) cells.

Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. 

Host Cell Line: Jurkat

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Uniprot: Q96BF3

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS78323
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78323
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human