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Applications: Screen Vγ9Vδ2 TCR agonist antibodies.Positive control for Vγ9Vδ2 TCR evaluation and optimization of experimental conditions.
Background: γδ TCRs (T cell receptors), αβ TCRs, and antibodies, result from gene rearrangements and offer the immune system the possibility to recognize several different types of antigens. γδ TCRs recognize antigens in a similar way to antibodies, being able to recognize full protein antigens and being independent on antigen binding to the MHC (major histocompatibility complex). Examples of such antigens include pyrophosphomonoesters from Mycobacterium tuberculosis and E. Coli, or glycoprotein I from Herpes simplex. γδ TCRs are cell type specific, with Vγ9Vδ2 being present in T cells and corresponding to about 5% of the T cell population in blood. Vγ9Vδ2 T cells are involved mostly in immune responses to pathogens and long-term modulation of inflammation, and can recognize non-peptide phosphor-antigens, alkylamines and synthetic aminobisphosphonates. Vγ9Vδ2 T cells are being studied for the treatment of solid tumors and hematological disorders and are becoming a highly promising cancer therapy. Further studies on how best to utilize Vγ9Vδ2 T cells, and methods to enhance their presence, will open new therapeutic avenues for cancer and infections.
Description: Vγ9Vδ2 TCR NFAT-Luciferase Reporter Jurkat Cell Line was generated from T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (#78556) by overexpression of human Vγ9Vδ2 TCR using lentiviral transduction (with Vγ9Vδ2 TCR Lentivirus, #78985).This cell line was functionally validated with a TCR Vδ2 agonist antibody.
Host Cell Line: Jurkat (clone E6-1)
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Allison T. and Garboczi D., 2002 Molecular Immunology 38 (14): 1051-1061.
Sawaisorn P., et al., 2024 Scientific Reports 14: 1291.
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