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Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for Use With CD19 / BCMA / Firefly Luciferase - CHO Recombinant Cell Line (BPS Bioscience, #78030)
Formulation: BPS Growth Media is formulated through BPS Thaw Media combined with antibiotics for maintenance of selective markers in the stable cell line.
Growth Medium 3K (BPS Bioscience #78041): Thaw medium 3 (BPS Bioscience #60186) plus 1000 µg/ml Geneticin, 5 µg/ml Puromycin (InvivoGen, #ant-pr-1) and 500 µg/ml of Hygromycin B to ensure recombinant expression.
o Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.
Mycoplasma Testing: Sterile filtered (0.2 µm) and Mycoplasma-free. Each lot is tested for growth of recombinant cells. All components are low endotoxin. FBS is ISIA-certified and sourced from the United States of America.
Storage Stability: Upon receipt, the medium should be stored in the dark at 4°C. Avoid repeated freeze/thaw cycles. Do not exceed 37°C. When stored in the dark at 4°C, the product is stable for six months after first thaw.
Supplied As: Liquid
Biosafety Level: Not applicable (BSL-1)
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