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Application: Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context
Background: L-Trp is an essential amino acid necessary for protein synthesis in mammaliancells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate andadaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressivemicroenvironment by starving immune cells of L-Trp and releasing degradation products of LTrpthat have immunosuppressive functions. IDO1 is upregulated in many tumors, providingcancer cells with an avenue for immune evasion.
Description: The IDO1 Cellular Activity QuickDetect™ Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1- expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of tryptophan 2,3 dioxygenase (IDO1)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Storage Stability: Stable for at least 6 months when stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
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