SF Cell Line 4D-Nucleofector™ LV Kit XL

The SF Cell Line 4D-Nucleofector™ LV Kit XL* is suited for larger scale transfection of cell lines in the LV Nucleocuvette™ Cartridge format when working with the 4D-Nucleofector™ LV Unit. With the LV Nucleocuvette™ Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector™ System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells).

Content:
•22.5 mL SF Cell Line Nucleofector™ Solution
•5 mL Supplement 1
•50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
•1 x LV Nucleocuvette™ Cartridge
•2 x 4D-Nucleofector™ LV Reservoir

• SKU: LONV4LC-2020
• Manufacturer: Lonza
• Manufacturer SKU: V4LC-2020
• Package Unit: ca. 10 million cells PEL
• Quantity Unit: PAK
• Type: Nucleofection Kit