The Cell Line Optimization 4D-Nucleofector™ X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector™ X Unit.
With the unique capability of the 4D-Nucleofector™ X Unit to address individual wells of a 16-well Nucleocuvette™ Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well Nucleocuvette™ Strips and our pmaxGFPTM Vector as positive control, our three Cell Line Nucleofector™ Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette™ Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.
Content:
•0.675 mL Nucleofector™ Solution SE
•0.675 mL Nucleofector™ Solution SF
•0.675 mL Nucleofector™ Solution SG
•3 x 0.15 mL Supplement 1
•50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0) ,
•4 x 20 µL 16-well Nucleocuvette™ Strips
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