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Encompassing Amino Acids: 2-442(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to Cereblon Binding Assay Kit (BPS Bioscience #79899) with CRBN titrated from 2400 ng/rxn - 0 ng/rxn incubated for 90 minutes at room temperature. Fluorescence polarization measured at ex635 nm, em682 nm.
Description: Recombinant human cereblon (CRBN), full length, encompassing amino acids 2-422(end). This construct contains an N-terminal Flag-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 µg ml FLAG peptide
Genbank: NM_016302
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q96SW2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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