Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for screening small molecular inhibitors or PROTACs for drug discovery and HTS applications.
Contraindications: Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Description: The Cereblon Ubiquitination Homogeneous Assay Kit is designed to measure Cereblon auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and proper A-screen beads to complete the pairing. This homogeneous assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
Storage Stability: At least six months from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions
Warnings: Thalidomide is known to cause severe birth defects in humans. It is very important to use all appropriate precautions when handling this compound.
Biosafety Level: Not applicable (BSL-1)
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